Differentiation of Mycobacterium tuberculosis complex by PCR amplification of genomic regions of difference.
نویسندگان
چکیده
Differentiation of members of the Mycobacterium tuberculosis complex by conventional mycobacteriological methods is time consuming, making surveillance of species-specific disease difficult. A two-step, multiplex polymerase chain reaction (PCR) method based on genomic regions of difference (RD1, RD1(mic), RD2(seal), RD4, RD9 and RD12) was developed for the differentiation of M. canettii, M. tuberculosis, M. africanum, M. microti, M. pinnipedii, M. caprae, M. bovis and M. bovis BCG. The size of the respective multiplex PCR amplification products corresponded to the presence of the different M. tuberculosis complex members. This method allows for rapid differentiation, making it suitable for routine laboratories and surveillance purposes.
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ورودعنوان ژورنال:
- The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease
دوره 10 7 شماره
صفحات -
تاریخ انتشار 2006